- Title
- Pyrosequencing analysis of bacterial diversity in soils contaminated long-term with PAHs and heavy metals: implications to bioremediation
- Creator
- Kuppusamy, Saranya; Thavamani, Palanisami; Megharaj, Mallavarapu; Venkateswarlu, Kadiyala; Lee, Yong Bok; Naidu, Ravi
- Relation
- Journal of Hazardous Materials Vol. 317, p. 169-179
- Publisher Link
- http://dx.doi.org/10.1016/j.jhazmat.2016.05.066
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2016
- Description
- Diversity, distribution and composition of bacterial community of soils contaminated long-term with both polycyclic aromatic hydrocarbons (PAHs) and heavy metals were explored for the first time following 454 pyrosequencing. Strikingly, the complete picture of the Gram positive (+ve) and Gram negative (-ve) bacterial profile obtained in our study illustrates novel postulates that include: (1) Metal-tolerant and PAH-degrading Gram -ves belonging to the class Alphaproteobacteria persist relatively more in the real contaminated sites compared to Gram +ves, (2) Gram +ves are not always resistant to heavy metal toxicity, (3) Stenotrophomonas followed by Burkholderia and Pseudomonas are the dominant genera of PAH degraders with high metabolic activity in long-term contaminated soils, (4) Actinobacteria is the predominant group among the Gram +ves in soils contaminated with high molecular weight PAHs that co-exist with toxic heavy metals like Pb, Cu and Zn, (5) Microbial communities are nutrient-driven in natural environments and (6) Catabolically potential Gram +/-ves with diverse applicability to remediate the real contaminated sites evolve eventually in the historically-polluted soils. Thus, the most promising indigenous Gram +/-ve strains from the long-term contaminated sites with increased catabolic potential, enzymatic activity and metal tolerance need to be harnessed for mixed contaminant cleanups.
- Subject
- pyrosequencing; PAH degraders; heavy metals; mixed contamination; microbial community
- Identifier
- http://hdl.handle.net/1959.13/1323964
- Identifier
- uon:24925
- Identifier
- ISSN:0304-3894
- Language
- eng
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